CAS 9025-65-4 Nr Benzonase Nuclease

Khoom Taw Qhia thiab Daim Ntawv Thov:
     Benzonase nuclease yog ib qho genetic engineered endonuclease muab los ntawm Serratia marcescens, nrog ib tug molecular hnyav ntawm 26kD thiab CAS accession tooj 9025-}65-4 [4-5]. Cov enzyme no tuaj yeem ua rau txhua hom DNA thiab RNA (xws li ib leeg, ob txoj hlua khi, kab, thiab ncig) nyob rau hauv lub xub ntiag ntawm Mg ² +, ua rau 3-5 lub hauv paus ntev 5 '- monophosphate oligonucleotides uas ua raws li FDA nucleic acid kev kho kab mob sib kis. Cov tshuaj tiv thaiv zoo tshaj plaws yog pH 6-10, qhov kub thiab txias 35-44 degree, kev ua haujlwm ntawm 1 × 10 ⁶ U / mg protein, thiab ua siab ntev rau denaturing agents xws li 4M urea.
Benzonase nuclease yog siv rau hauv kev ua kom huv ntawm cov recombinant proteins, kev npaj cov txheej txheem suav nrog, thiab tshem tawm cov tswv cell DNA hauv cov tshuaj tiv thaiv los ntawm kev txo cov qauv viscosity thiab txhim kho kev sib cais centrifugal thaum ua ke nrog lysates xws li BacReady Protein Extraction Solution. Lub enzyme tseem ua lub luag haujlwm tseem ceeb hauv kev sim xws li nucleoprotein extraction, 2D gel electrophoresis thiab Western Blot, thiab tuaj yeem ua tsis tau los ntawm EDTA reversible inhibition lossis NaOH / kev kho kub kub [6] [8]. Cov khoom specifications muaj li ntawm 25KU rau 10000KU thiab yuav tsum tau muab cia rau hauv qhov tsaus ntuj ntawm -20 degree.

Synonyms:

NUCLEASE
ENDONUCLEASE

Ultra Nuclease

Nuclease S1
BENZONASE (R)
MUNG BEAN NUCLEASE

Nuclease ntawm Serratia marcescens (Nuclease)

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